EMX2C9100-23B Datasheet, PDF (6/8 Pages) Hamamatsu Corporation – Multiply faster

Selectable Readout Modes
Select a readout mode for optimal image acquisition based on the sample brightness or desired frame rate or exposure time.
EM-CCD readout
For short exposure, high sensitivity imaging
Sample of EM-CCD readout
Confocal calcium ion imaging of HeLa cells expressing yellow
Chameleon 3.6. This image shows changes of histamine stimulated
calcium ion with two Z positions and four time lapse.
Objective lens: 100×
EM gain: 300×
Exposure time: 100 ms
Confocal unit:
CSU by Yokogawa Electric Co.
CFP/YFP FRET:
2 wavelength imaging,
W-view optics A8509
Z scan: 19 slices/2.5 s
Piezoelectric Z stage
Data courtesy of:
Dr. Kenji Nagai, Dr. Kenta Saito
Hokkaido Univ.
Nikon imaging center
Normal-CCD readout
For high dynamic range imaging
Sample of Normal-CCD readout
Luminescence imaging of HeLa cells expressing Renilla Luciferase.
Objective lens: UApo/340 20×
Exposure time: 5 minutes
Cooling method: Water cooled
(-80 ˚C)
Binning: 2×2
An often overlooked benefit of EM-CCD technology is the ability to utilize the camera as a standard CCD. In non-EM
mode, there is no effect of excess noise, and the large full well capacity and high dynamic range are ideal for bright light
applications that have large intrascene dynamic range. The ImagEM X2 provides a low read noise non-EM mode that
can be an ideal choice for such applications.
Photon Imaging Mode
This is a unique technology to improve image quality
at very low light level to overcome the limitation of
excess noise factor from the electron multiplying
process. This mode is most useful for signal levels at
which maximum EM gain has no apparent signal or
very little signal. The mode preserves quantitative
linear signal output and also improves spatial
resolution at very low light levels.
Sample of photon imaging mode
Fluorescence beads imaged with reduced excitation light intensity.
Photon imaging mode OFF
Photon imaging mode ON
(Exposure time: 30.5 ms, EM gain: 1200×)
On-board Image Processing
The following real time processing functions are available.
Background subtraction
Effective for reducing fluorescence in image backgrounds.
Shading correction
This feature corrects the shading or uneven illumination in microscope images or other illumination systems.
Recursive filter
This feature provides random noise elimination in an image by weighted time based averaging.
Frame averaging
This feature provides noise elimination in an image by simple frame averaging and less “afterimage” effect than the recursive filter.
Spot noise reducer
This image processing function operates on random spots of intensity by comparing incoming images and eliminating signals
that meet the criteria for noise in one image but not in others. This processing eliminates noise elements like cosmic rays.