SC-12061 Datasheet, PDF (1/1 Pages) –

SANTA CRUZ BIOTECHNOLOGY, INC.
Aldolase A (C-16): sc-12061
The Power to Question
BACKGROUND
Fructose 1,6-bisphosphate Aldolase catalyses the reversible condensation of
glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate.
Fructose 1,6-bisphosphate Aldolase exists as three forms, the muscle-specif-
ic Aldolase A, the liver-specific Aldolase B, and the brain-specific Aldolase
C. Aldolase A, B, and C arose from a common ancestral gene, from which
Aldolase B first diverged. Aldolase A is one of the most highly conserved
enzymes known, with only about 2% of the residues changing per 100 mil-
lion years. Aldolase B is regulated by the hormones insulin and glucagon
and has been implicated in hereditary fructose intolerance disease. Aldolase
C is 36 kDa polypeptide that is exclusively expressed in Purkinje cells.
Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes
or bands that run from anterior to posterior across the cerebellum and alter-
nate with bands of Aldolase C-negative Purkinje cells.
REFERENCES
1. Caffe, A.R., et al. 1994. Distribution of Purkinje cell-specific zebrin-II/
Aldolase C immunoreactivity in the mouse, rat, rabbit, and human retina.
J. Comp. Neurol. 348: 291-297.
2. Lannoo, M.J., et al. 1997. A search for primitive Purkinje cells: zebrin II
expression in sea lampreys (Petromyzon marinus). Neurosci. Lett. 237:
53-55.
3. Walther, E.U., et al. 1998. Genomic sequences of Aldolase C (zebrin II)
direct lacZ expression exclusively in non-neuronal cells of transgenic mice.
Proc. Natl. Acad. Sci. USA 95: 2615-2620.
4. Dehnes, Y., et al. 1998. The glutamate transporter EAAT4 in rat cerebellar
Purkinje cells: a glutamate-gated chloride channel concentrated near the
synapse in parts of the dendritic membrane facing astroglia. J. Neurosci.
18: 3606-3619.
5. Izzo, P., et al. 1988. Human Aldolase A gene. Structural organization and
tissue-specific expression by multiple promoters and alternate mRNA pro-
cessing. Eur. J. Biochem. 174: 569-578.
6. Freemont, P.S., et al. 1988. The complete amino acid sequence of human
skeletal-muscle fructose-bisphosphate Aldolase. Biochem. J. 249: 779-788.
CHROMOSOMAL LOCATION
Genetic locus: ALDOA (human) mapping to 16q22-q24; Aldoa (mouse)
mapping to 7 F4.
SOURCE
Aldolase A (C-16) is an affinity purified goat polyclonal antibody raised
against a peptide mapping near the C-terminus of Aldolase A of human
origin.
APPLICATIONS
Aldolase A (C-16) is recommended for detection of Aldolase A of mouse,
rat and human origin by Western Blotting (starting dilution 1:200, dilution
range 1:100-1:1000), immunoprecipitation [1–2 µg per 100–500 µg of total
protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50,
dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30,
dilution range 1:30-1:3000).
Suitable for use as control antibody for Aldolase A siRNA (h): sc-29664 and
Aldolase A siRNA (m): sc-29665.
Molecular Weight of Aldolase A: 40 kDa.
Positive Controls: Sol8 cell lysate: sc-2249, RAW 264.7 whole cell lysate:
sc-2211 or A-673 cell lysate: sc-2414.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey anti-
goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip-
itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/ 2.0 ml).
3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution
range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range:
1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941.
DATA
52 K -
35 K -
30 K -
< Aldolase A
Aldolase A (C-16): sc-12061. Western blot analysis of
Aldolase A expression in KNRK whole cell lysate.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
PRODUCT
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and
0.1% gelatin.
Blocking peptide available for competition studies, sc-12061 P, (100 µg pep-
tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).
PROTOCOLS
See our web site at www.scbt.com or our catalog for detailed protocols
and support products.
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax [phone] Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com