PKD-MAP3K14-V1 Datasheet, PDF (1/5 Pages) –

Certificate of Analysis
pKD-MAP3K14-v1
(mammalian MAP3K14 siRNA expression plasmid)
Catalog # 62-091
Lot # 29008
Product Description: Transfection grade mammalian
expression plasmid containing human MAP3K14
sequence that when expressed forms a short-hairpin
RNA (shRNA), which gets processed into a MAP3K14
siRNA. The expression of the shRNA is under the
control of the human H1 RNA polymerase III promoter
and is terminated as illustrated.
Gene Target GenBank Accession Numbers:
NM_003954.
Species of Gene Target: Human.
Storage and Stability: Stable for 5 years at either 4°C
or -20°C from date of shipment.
48 Barn Road • Lake Placid, NY 12946
Technical Support: T: 800 548-7853 • F: 518 523-4513
email: [e-mail]
Sales Department: T: 800 233-3991 • F: 781 890-7738
Licensing Dept.: 800 310-4659
www.upstate.com
Formulation: 5µg affinity purified DNA eluted and
packaged aseptically in 50µl of 10mM Tris-HCl, pH 8.0,
1mM EDTA. The final concentration is 100ng/µl.
Guarantee: This product is guaranteed to knockdown
the intended target mRNA by at least 70% (as
compared to pKD-NegCon-v1) when transfected into
HeLa cells following the protocol stated on page 4.
Related Products (with catalog number):
pKD plasmid (62-001)
pKD-NegCon-v1 (62-002)
pKD-MAP3K14-v4 (62-092)
Alternate Nomenclature: NIK, HS, HSNIK, and
FTDCR1B.
Terms of Use
Opening and use of this product means that the purchaser agrees with the following conditions: The pKD
family of plasmids may not be freely distributed, resold, modified for resale or used to manufacture commercial
products without prior written approval from Upstate USA, Inc. If you do not agree with these conditions, please return
product to Upstate USA, Inc. for a full refund.
FOR RESEARCH USE ONLY
NOT FOR USE IN HUMANS
Quality Control Testing
Restriction Enzyme Digest: This lot of DNA was cut with
EcoRI and SpeI, which generated the expected
fragments.
Plasmid Transfection and Quantitative RT-PCR Analysis:
Representative data shown below.
Q-RT-PCR Analysis: The efficiency of the pKD plasmid
to knockdown mRNA levels was measured by
quantitative RT-PCR using Taqman™ probes. Briefly,
HeLa cells were transfected (see protocol page 4) with
the gene specific pKD plasmid and RNA was harvested
48 hours post-transfection. The reduction in mRNA
expression was determined by comparison to RNA
harvested from cells transfected with the negative control
plasmid (pKD-NegCon-v1). Results were normalized by
measuring GAPDH expression levels.
Sequencing: The shRNA insert was verified by
automated, PCR-based, dideoxy chain-terminating
sequencing on the template plasmid.
Purity: The OD₂₆₀/OD₂₈₀ for this lot of DNA is 1.85.
pKD Knockdown Efficiency
120
100
80
60
40
20
0
pKD-NegCon-v1
pKD-MAP3K14-v1 pKD-MAP3K14-v4