SC-5610 Datasheet, PDF (1/1 Pages) –

Santa Cruz Biotechnology, Inc.
cyclin C (H-184): sc-5610
SANTA CRUZ
BIOTECHNOLOGY
BACKGROUND
The proliferation of eukaryotic cells is controlled at specific points
in the cell cycle, particularly at the G1 to S and the G2 to
M transitions. It is well established that the Cdc2 p34-cyclin B
protein kinase plays a critical role in the G2 to M transition (1)
while cyclin A associates with Cdk2 p33 and functions in S phase.
Considerable effort directed towards the identification of G1
cyclins has led to the isolation of cyclin D, cyclin C and
cyclin E (2-4). Cyclin D corresponds to a putative human
oncogene designated PRAD1 which maps at the site of the BCL1
rearrangement in certain lymphomas and leukemias (5). Cyclin C
complexes with the cyclin dependent kinase, Cdk8 (6). The
cyclin C/Cdk8 complex has been shown to have kinase activity
toward the carboxy terminal domain of RNA polymerase II. Two
complexes have been identified which contain cyclin C/Cdk8. A
complex of over 500 kDa has been found to contain the large
subunit of RNA polymerase II. A smaller complex of 170 kDa has
also been identified (6).
SOURCE
Cyclin C (H-184) is a rabbit polyclonal antibody raised against a
recombinant protein corresponding to amino acids 120-303
mapping at the carboxy terminus of cyclin C of human origin.
PRODUCT
Each vial contains 200 µg IgG in 1.0 ml of PBS containing
0.1% sodium azide and 0.2% gelatin.
STORAGE
Store at 4° C, do not freeze; stable for one year from the date of
shipment.
SPECIFICITY
Cyclin C (H-184) reacts with cyclin C of mouse, rat and human
origin by Western blotting, immunoprecipitation and immuno-
histochemistry.
Recommended dilution range for Western blot analysis:
1:200–1:1000. Recommended starting dilution: 1:200.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
REFERENCES
1. Draetta, G. 1990. Cell cycle control in eukaryotes: molecular
mechanisms of cdc2 activation. Trends Biol. Sci. 15: 378-383.
2. Xiong, Y., Connolly, T., Futcher, B., and Beach, D. 1991.
Human D-Type cyclin. Cell 65: 691-699.
3. Xiong, Y., Menninger, J., Beach, D., and Ward, D. C. 1992.
Molecular cloning and chromosomal mapping of CCND genes
encoding human D-type cyclins. Genomics 13: 575-584.
4. Lew, D.J., Dulic, V., and Reed, S.I. 1991. Isolation of three
novel human cyclins by rescue of G1 cyclin (CIn) Function in
yeast. Cell 66: 1197-1206.
5. Won, K., Xiong, Y., Beach, D., and Gilman, M.Z. 1992.
Growth-regulated expression of D-type cyclin genes in human
diploid fibroblasts. Proc. Natl. Acad. Sci. USA 89: 9910-9914.
6. Rickert, P., Seghezzi, W., Shanahan, F., Cho, H., and
Lees, E. 1996. Cyclin C/Cdk8 is a novel CTD kinase associated
with RNA polymerase II. Oncogene 12: 2631-2640.
U.S. 1.800.457.3801 • 831.457.3800 • fax [phone] • Europe [phone] • 49 (0)6221 4503 0 • fax [phone] • www.scbt.com
June 2000
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